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viability reagent cck 8  (Dojindo Labs)


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    Structured Review

    Dojindo Labs viability reagent cck 8
    Viability Reagent Cck 8, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 99/100, based on 59887 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cck+8+cell+viability+assay+kit/Cell+Counting+Kit-8/pm42144083-154-12-16
    Average 99 stars, based on 59887 article reviews
    viability reagent cck 8 - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    CCK-8 Assay:

    Article Title: Tea tree oil in inhibiting oral cariogenic bacterial growth an in vivo study for managing dental caries
    Article Snippet: .. The CCK-8 Cell Viability Assay Kit (CK04-01, Dojindo) was used to evaluate the cell viability following the manufacturer’s instructions. .. After incubation with CCK-8 solution for 2 h at 37 °C, we measured the absorbance at 450 nm using a microplate reader (Varioskan LUX Multimode Microplate Reader, Thermo Scientific).

    Article Title: BFGF mitigates CTX-induced ovarian cytotoxicity via SERPINE1/HIF-1 and Nrf-2/HO-1 signaling pathways
    Article Snippet: .. DMEM/F12 Medium: Gibco, Thermo Fisher Scientific (Catalog No. 11330032); Fetal Bovine Serum (FBS): Gibco, Thermo Fisher Scientific (Catalog No. 10099141); Penicillin-Streptomycin: Gibco, Thermo Fisher Scientific (Catalog No. 15140122); Cyclophosphamide (CTX): Sigma-Aldrich (Catalog No. C0768); Basic Fibroblast Growth Factor (BFGF): PeproTech (Catalog No. 100-18B); BFGF Inhibitor: R&D Systems (Catalog No. 7183-FB); CCK-8 Cell Viability Assay Kit: Dojindo Laboratories (Catalog No. CK04); Annexin V-FITC/PI Apoptosis Detection Kit: BD Biosciences (Catalog No. 556547); JC-1 Mitochondrial Membrane Potential Assay Kit: Abcam (Catalog No. ab113850); TUNEL Assay Kit: Roche (Catalog No. 11684795910); RIPA Lysis Buffer: Thermo Fisher Scientific (Catalog No. 89901); Protease and Phosphatase Inhibitor Cocktail: Sigma-Aldrich (Catalog No. P5726); PVDF Membranes: Millipore (Catalog No. IPVH00010); Enhanced Chemiluminescence (ECL) Detection Kit: Thermo Fisher Scientific (Catalog No. 32106); SERPINE1 Antibody: Abcam (Catalog No. ab125687); HIF-1α Antibody: Cell Signaling Technology (Catalog No. 3716); Nrf-2 Antibody: Abcam (Catalog No. ab137550); HO-1 Antibody: Cell Signaling Technology (Catalog No. 43966); Cyclin D1 Antibody: Cell Signaling Technology (Catalog No. 2978); CDK4 Antibody: Cell Signaling Technology (Catalog No. 12790); TGF-β Antibody: Abcam (Catalog No. ab92486); FSH Antibody: Abcam (Catalog No. ab151564); ER Antibody: Abcam (Catalog No. ab16660); β-actin Antibody: Cell Signaling Technology (Catalog No. 4970); HRP-Conjugated Secondary Antibodies: Cell Signaling Technology (Catalog No. 7074 for anti-rabbit, 7076 for anti-mouse); TRIzol Reagent: Thermo Fisher Scientific (Catalog No. 15596026); cDNA Synthesis Kit: Thermo Fisher Scientific (Catalog No. 4368814); SYBR Green PCR Master Mix: Thermo Fisher Scientific (Catalog No. A25742); Triton X-100: Sigma-Aldrich (Catalog No. T8787); Bovine Serum Albumin (BSA): Sigma-Aldrich (Catalog No. A9418); DAPI Stain: Thermo Fisher Scientific (Catalog No. D1306); Fluorescent Secondary Antibodies: Thermo Fisher Scientific (Catalog Nos. .. A-11001 and A-11008); Illumina TruSeq RNA Sample Preparation Kit: Illumina (Catalog No. RS-122-2001); Agilent RNA 6000 Nano Kit: Agilent Technologies (Catalog No. 5067 − 1511); Microplate reader (Super Max3100); Fluorescence microscope (Axio Vert A1);Flow cytometer (FASVERSE).

    Article Title: Immune and Stem Cell-Mediated Bactericidal Amplification and Bone Remodeling for Infection Clearance and Osteointegration.
    Article Snippet: .. CCK-8 cell viability assay kit was urchased from Dojindo Molecular Technologies (Kumamoto, apan). ..

    Article Title: Hyperglycemia drives lmmune evasion in colorectal cancer through VISTA stabilization
    Article Snippet: GST-ERK2 (ab155812), ATP-γ-S, and p-Nitrobenzylmesylate (PNBM) were purchased from Abcam. .. The Lipo8000 transfection reagent was purchased from Beyotime, while the CCK-8 cell viability assay kit was acquired from DOJINDO. .. Anti-FLAG ® M2 Magnetic Beads, S02 and streptozotocin (STZ) were supplied by Sigma.

    Article Title: Hyperglycemia drives lmmune evasion in colorectal cancer through VISTA stabilization.
    Article Snippet: GST‐ERK2 129 (ab155812), ATP-γ-S, and p-Nitrobenzylmesylate (PNBM) were purchased 130 from Abcam. .. The Lipo8000 transfection reagent was purchased from 131 Beyotime, while the CCK-8 cell viability assay kit was acquired from 132 DOJINDO. .. Anti-FLAG® M2 Magnetic Beads, S02 and streptozotocin (STZ) 133 were supplied by Sigma.

    Article Title: Tea tree oil in inhibiting oral cariogenic bacterial growth an in vivo study for managing dental caries.
    Article Snippet: .. The CCK-8 Cell Viability Assay Kit (CK04-01, Dojindo) was used to evaluate the cell viability following the manufacturer’s instructions. .. After incubation with CCK-8 solution for 2 h at 37 °C, we measured the absorbance at 450 nm using a microplate reader (Varioskan LUX Multimode Microplate Reader, Thermo Scientific).

    Article Title: Composition analysis of extracellular vesicle-like particles derived from Hedyotis diffusa Willd and their effects on apoptosis in hepatocellular carcinoma Huh-7 cells.
    Article Snippet: As significant facilitators of intercellular communication, extracellular vesicles transport various bioactive molecules that contribute to the modulation of the tumor microenvironment.. CHM-EVLPs can markedly influence the biological behavior of tumor cells, exhibiting antitumor effects.. In this study, EV-like particles derived from Hedyotis diffusa Willd (HDW-EVLPs) were successfully isolated using the 3D FloTrix® vivaEXO exosome harvesting system.

    Viability Assay:

    Article Title: Tea tree oil in inhibiting oral cariogenic bacterial growth an in vivo study for managing dental caries
    Article Snippet: .. The CCK-8 Cell Viability Assay Kit (CK04-01, Dojindo) was used to evaluate the cell viability following the manufacturer’s instructions. .. After incubation with CCK-8 solution for 2 h at 37 °C, we measured the absorbance at 450 nm using a microplate reader (Varioskan LUX Multimode Microplate Reader, Thermo Scientific).

    Article Title: BFGF mitigates CTX-induced ovarian cytotoxicity via SERPINE1/HIF-1 and Nrf-2/HO-1 signaling pathways
    Article Snippet: .. DMEM/F12 Medium: Gibco, Thermo Fisher Scientific (Catalog No. 11330032); Fetal Bovine Serum (FBS): Gibco, Thermo Fisher Scientific (Catalog No. 10099141); Penicillin-Streptomycin: Gibco, Thermo Fisher Scientific (Catalog No. 15140122); Cyclophosphamide (CTX): Sigma-Aldrich (Catalog No. C0768); Basic Fibroblast Growth Factor (BFGF): PeproTech (Catalog No. 100-18B); BFGF Inhibitor: R&D Systems (Catalog No. 7183-FB); CCK-8 Cell Viability Assay Kit: Dojindo Laboratories (Catalog No. CK04); Annexin V-FITC/PI Apoptosis Detection Kit: BD Biosciences (Catalog No. 556547); JC-1 Mitochondrial Membrane Potential Assay Kit: Abcam (Catalog No. ab113850); TUNEL Assay Kit: Roche (Catalog No. 11684795910); RIPA Lysis Buffer: Thermo Fisher Scientific (Catalog No. 89901); Protease and Phosphatase Inhibitor Cocktail: Sigma-Aldrich (Catalog No. P5726); PVDF Membranes: Millipore (Catalog No. IPVH00010); Enhanced Chemiluminescence (ECL) Detection Kit: Thermo Fisher Scientific (Catalog No. 32106); SERPINE1 Antibody: Abcam (Catalog No. ab125687); HIF-1α Antibody: Cell Signaling Technology (Catalog No. 3716); Nrf-2 Antibody: Abcam (Catalog No. ab137550); HO-1 Antibody: Cell Signaling Technology (Catalog No. 43966); Cyclin D1 Antibody: Cell Signaling Technology (Catalog No. 2978); CDK4 Antibody: Cell Signaling Technology (Catalog No. 12790); TGF-β Antibody: Abcam (Catalog No. ab92486); FSH Antibody: Abcam (Catalog No. ab151564); ER Antibody: Abcam (Catalog No. ab16660); β-actin Antibody: Cell Signaling Technology (Catalog No. 4970); HRP-Conjugated Secondary Antibodies: Cell Signaling Technology (Catalog No. 7074 for anti-rabbit, 7076 for anti-mouse); TRIzol Reagent: Thermo Fisher Scientific (Catalog No. 15596026); cDNA Synthesis Kit: Thermo Fisher Scientific (Catalog No. 4368814); SYBR Green PCR Master Mix: Thermo Fisher Scientific (Catalog No. A25742); Triton X-100: Sigma-Aldrich (Catalog No. T8787); Bovine Serum Albumin (BSA): Sigma-Aldrich (Catalog No. A9418); DAPI Stain: Thermo Fisher Scientific (Catalog No. D1306); Fluorescent Secondary Antibodies: Thermo Fisher Scientific (Catalog Nos. .. A-11001 and A-11008); Illumina TruSeq RNA Sample Preparation Kit: Illumina (Catalog No. RS-122-2001); Agilent RNA 6000 Nano Kit: Agilent Technologies (Catalog No. 5067 − 1511); Microplate reader (Super Max3100); Fluorescence microscope (Axio Vert A1);Flow cytometer (FASVERSE).

    Article Title: Immune and Stem Cell-Mediated Bactericidal Amplification and Bone Remodeling for Infection Clearance and Osteointegration.
    Article Snippet: .. CCK-8 cell viability assay kit was urchased from Dojindo Molecular Technologies (Kumamoto, apan). ..

    Article Title: Hyperglycemia drives lmmune evasion in colorectal cancer through VISTA stabilization
    Article Snippet: GST-ERK2 (ab155812), ATP-γ-S, and p-Nitrobenzylmesylate (PNBM) were purchased from Abcam. .. The Lipo8000 transfection reagent was purchased from Beyotime, while the CCK-8 cell viability assay kit was acquired from DOJINDO. .. Anti-FLAG ® M2 Magnetic Beads, S02 and streptozotocin (STZ) were supplied by Sigma.

    Article Title: Hyperglycemia drives lmmune evasion in colorectal cancer through VISTA stabilization.
    Article Snippet: GST‐ERK2 129 (ab155812), ATP-γ-S, and p-Nitrobenzylmesylate (PNBM) were purchased 130 from Abcam. .. The Lipo8000 transfection reagent was purchased from 131 Beyotime, while the CCK-8 cell viability assay kit was acquired from 132 DOJINDO. .. Anti-FLAG® M2 Magnetic Beads, S02 and streptozotocin (STZ) 133 were supplied by Sigma.

    Article Title: Tea tree oil in inhibiting oral cariogenic bacterial growth an in vivo study for managing dental caries.
    Article Snippet: .. The CCK-8 Cell Viability Assay Kit (CK04-01, Dojindo) was used to evaluate the cell viability following the manufacturer’s instructions. .. After incubation with CCK-8 solution for 2 h at 37 °C, we measured the absorbance at 450 nm using a microplate reader (Varioskan LUX Multimode Microplate Reader, Thermo Scientific).

    Article Title: Composition analysis of extracellular vesicle-like particles derived from Hedyotis diffusa Willd and their effects on apoptosis in hepatocellular carcinoma Huh-7 cells.
    Article Snippet: As significant facilitators of intercellular communication, extracellular vesicles transport various bioactive molecules that contribute to the modulation of the tumor microenvironment.. CHM-EVLPs can markedly influence the biological behavior of tumor cells, exhibiting antitumor effects.. In this study, EV-like particles derived from Hedyotis diffusa Willd (HDW-EVLPs) were successfully isolated using the 3D FloTrix® vivaEXO exosome harvesting system.

    Article Title: Immune and Stem Cell‐Mediated Bactericidal Amplification and Bone Remodeling for Infection Clearance and Osteointegration
    Article Snippet: Secondary fluorescent antibodies and DAPI were obtained from Invitrogen (Carlsbad, CA, USA). .. CCK‐8 cell viability assay kit was purchased from Dojindo Molecular Technologies (Kumamoto, Japan). ..

    Membrane:

    Article Title: BFGF mitigates CTX-induced ovarian cytotoxicity via SERPINE1/HIF-1 and Nrf-2/HO-1 signaling pathways
    Article Snippet: .. DMEM/F12 Medium: Gibco, Thermo Fisher Scientific (Catalog No. 11330032); Fetal Bovine Serum (FBS): Gibco, Thermo Fisher Scientific (Catalog No. 10099141); Penicillin-Streptomycin: Gibco, Thermo Fisher Scientific (Catalog No. 15140122); Cyclophosphamide (CTX): Sigma-Aldrich (Catalog No. C0768); Basic Fibroblast Growth Factor (BFGF): PeproTech (Catalog No. 100-18B); BFGF Inhibitor: R&D Systems (Catalog No. 7183-FB); CCK-8 Cell Viability Assay Kit: Dojindo Laboratories (Catalog No. CK04); Annexin V-FITC/PI Apoptosis Detection Kit: BD Biosciences (Catalog No. 556547); JC-1 Mitochondrial Membrane Potential Assay Kit: Abcam (Catalog No. ab113850); TUNEL Assay Kit: Roche (Catalog No. 11684795910); RIPA Lysis Buffer: Thermo Fisher Scientific (Catalog No. 89901); Protease and Phosphatase Inhibitor Cocktail: Sigma-Aldrich (Catalog No. P5726); PVDF Membranes: Millipore (Catalog No. IPVH00010); Enhanced Chemiluminescence (ECL) Detection Kit: Thermo Fisher Scientific (Catalog No. 32106); SERPINE1 Antibody: Abcam (Catalog No. ab125687); HIF-1α Antibody: Cell Signaling Technology (Catalog No. 3716); Nrf-2 Antibody: Abcam (Catalog No. ab137550); HO-1 Antibody: Cell Signaling Technology (Catalog No. 43966); Cyclin D1 Antibody: Cell Signaling Technology (Catalog No. 2978); CDK4 Antibody: Cell Signaling Technology (Catalog No. 12790); TGF-β Antibody: Abcam (Catalog No. ab92486); FSH Antibody: Abcam (Catalog No. ab151564); ER Antibody: Abcam (Catalog No. ab16660); β-actin Antibody: Cell Signaling Technology (Catalog No. 4970); HRP-Conjugated Secondary Antibodies: Cell Signaling Technology (Catalog No. 7074 for anti-rabbit, 7076 for anti-mouse); TRIzol Reagent: Thermo Fisher Scientific (Catalog No. 15596026); cDNA Synthesis Kit: Thermo Fisher Scientific (Catalog No. 4368814); SYBR Green PCR Master Mix: Thermo Fisher Scientific (Catalog No. A25742); Triton X-100: Sigma-Aldrich (Catalog No. T8787); Bovine Serum Albumin (BSA): Sigma-Aldrich (Catalog No. A9418); DAPI Stain: Thermo Fisher Scientific (Catalog No. D1306); Fluorescent Secondary Antibodies: Thermo Fisher Scientific (Catalog Nos. .. A-11001 and A-11008); Illumina TruSeq RNA Sample Preparation Kit: Illumina (Catalog No. RS-122-2001); Agilent RNA 6000 Nano Kit: Agilent Technologies (Catalog No. 5067 − 1511); Microplate reader (Super Max3100); Fluorescence microscope (Axio Vert A1);Flow cytometer (FASVERSE).

    TUNEL Assay:

    Article Title: BFGF mitigates CTX-induced ovarian cytotoxicity via SERPINE1/HIF-1 and Nrf-2/HO-1 signaling pathways
    Article Snippet: .. DMEM/F12 Medium: Gibco, Thermo Fisher Scientific (Catalog No. 11330032); Fetal Bovine Serum (FBS): Gibco, Thermo Fisher Scientific (Catalog No. 10099141); Penicillin-Streptomycin: Gibco, Thermo Fisher Scientific (Catalog No. 15140122); Cyclophosphamide (CTX): Sigma-Aldrich (Catalog No. C0768); Basic Fibroblast Growth Factor (BFGF): PeproTech (Catalog No. 100-18B); BFGF Inhibitor: R&D Systems (Catalog No. 7183-FB); CCK-8 Cell Viability Assay Kit: Dojindo Laboratories (Catalog No. CK04); Annexin V-FITC/PI Apoptosis Detection Kit: BD Biosciences (Catalog No. 556547); JC-1 Mitochondrial Membrane Potential Assay Kit: Abcam (Catalog No. ab113850); TUNEL Assay Kit: Roche (Catalog No. 11684795910); RIPA Lysis Buffer: Thermo Fisher Scientific (Catalog No. 89901); Protease and Phosphatase Inhibitor Cocktail: Sigma-Aldrich (Catalog No. P5726); PVDF Membranes: Millipore (Catalog No. IPVH00010); Enhanced Chemiluminescence (ECL) Detection Kit: Thermo Fisher Scientific (Catalog No. 32106); SERPINE1 Antibody: Abcam (Catalog No. ab125687); HIF-1α Antibody: Cell Signaling Technology (Catalog No. 3716); Nrf-2 Antibody: Abcam (Catalog No. ab137550); HO-1 Antibody: Cell Signaling Technology (Catalog No. 43966); Cyclin D1 Antibody: Cell Signaling Technology (Catalog No. 2978); CDK4 Antibody: Cell Signaling Technology (Catalog No. 12790); TGF-β Antibody: Abcam (Catalog No. ab92486); FSH Antibody: Abcam (Catalog No. ab151564); ER Antibody: Abcam (Catalog No. ab16660); β-actin Antibody: Cell Signaling Technology (Catalog No. 4970); HRP-Conjugated Secondary Antibodies: Cell Signaling Technology (Catalog No. 7074 for anti-rabbit, 7076 for anti-mouse); TRIzol Reagent: Thermo Fisher Scientific (Catalog No. 15596026); cDNA Synthesis Kit: Thermo Fisher Scientific (Catalog No. 4368814); SYBR Green PCR Master Mix: Thermo Fisher Scientific (Catalog No. A25742); Triton X-100: Sigma-Aldrich (Catalog No. T8787); Bovine Serum Albumin (BSA): Sigma-Aldrich (Catalog No. A9418); DAPI Stain: Thermo Fisher Scientific (Catalog No. D1306); Fluorescent Secondary Antibodies: Thermo Fisher Scientific (Catalog Nos. .. A-11001 and A-11008); Illumina TruSeq RNA Sample Preparation Kit: Illumina (Catalog No. RS-122-2001); Agilent RNA 6000 Nano Kit: Agilent Technologies (Catalog No. 5067 − 1511); Microplate reader (Super Max3100); Fluorescence microscope (Axio Vert A1);Flow cytometer (FASVERSE).

    Lysis:

    Article Title: BFGF mitigates CTX-induced ovarian cytotoxicity via SERPINE1/HIF-1 and Nrf-2/HO-1 signaling pathways
    Article Snippet: .. DMEM/F12 Medium: Gibco, Thermo Fisher Scientific (Catalog No. 11330032); Fetal Bovine Serum (FBS): Gibco, Thermo Fisher Scientific (Catalog No. 10099141); Penicillin-Streptomycin: Gibco, Thermo Fisher Scientific (Catalog No. 15140122); Cyclophosphamide (CTX): Sigma-Aldrich (Catalog No. C0768); Basic Fibroblast Growth Factor (BFGF): PeproTech (Catalog No. 100-18B); BFGF Inhibitor: R&D Systems (Catalog No. 7183-FB); CCK-8 Cell Viability Assay Kit: Dojindo Laboratories (Catalog No. CK04); Annexin V-FITC/PI Apoptosis Detection Kit: BD Biosciences (Catalog No. 556547); JC-1 Mitochondrial Membrane Potential Assay Kit: Abcam (Catalog No. ab113850); TUNEL Assay Kit: Roche (Catalog No. 11684795910); RIPA Lysis Buffer: Thermo Fisher Scientific (Catalog No. 89901); Protease and Phosphatase Inhibitor Cocktail: Sigma-Aldrich (Catalog No. P5726); PVDF Membranes: Millipore (Catalog No. IPVH00010); Enhanced Chemiluminescence (ECL) Detection Kit: Thermo Fisher Scientific (Catalog No. 32106); SERPINE1 Antibody: Abcam (Catalog No. ab125687); HIF-1α Antibody: Cell Signaling Technology (Catalog No. 3716); Nrf-2 Antibody: Abcam (Catalog No. ab137550); HO-1 Antibody: Cell Signaling Technology (Catalog No. 43966); Cyclin D1 Antibody: Cell Signaling Technology (Catalog No. 2978); CDK4 Antibody: Cell Signaling Technology (Catalog No. 12790); TGF-β Antibody: Abcam (Catalog No. ab92486); FSH Antibody: Abcam (Catalog No. ab151564); ER Antibody: Abcam (Catalog No. ab16660); β-actin Antibody: Cell Signaling Technology (Catalog No. 4970); HRP-Conjugated Secondary Antibodies: Cell Signaling Technology (Catalog No. 7074 for anti-rabbit, 7076 for anti-mouse); TRIzol Reagent: Thermo Fisher Scientific (Catalog No. 15596026); cDNA Synthesis Kit: Thermo Fisher Scientific (Catalog No. 4368814); SYBR Green PCR Master Mix: Thermo Fisher Scientific (Catalog No. A25742); Triton X-100: Sigma-Aldrich (Catalog No. T8787); Bovine Serum Albumin (BSA): Sigma-Aldrich (Catalog No. A9418); DAPI Stain: Thermo Fisher Scientific (Catalog No. D1306); Fluorescent Secondary Antibodies: Thermo Fisher Scientific (Catalog Nos. .. A-11001 and A-11008); Illumina TruSeq RNA Sample Preparation Kit: Illumina (Catalog No. RS-122-2001); Agilent RNA 6000 Nano Kit: Agilent Technologies (Catalog No. 5067 − 1511); Microplate reader (Super Max3100); Fluorescence microscope (Axio Vert A1);Flow cytometer (FASVERSE).

    cDNA Synthesis:

    Article Title: BFGF mitigates CTX-induced ovarian cytotoxicity via SERPINE1/HIF-1 and Nrf-2/HO-1 signaling pathways
    Article Snippet: .. DMEM/F12 Medium: Gibco, Thermo Fisher Scientific (Catalog No. 11330032); Fetal Bovine Serum (FBS): Gibco, Thermo Fisher Scientific (Catalog No. 10099141); Penicillin-Streptomycin: Gibco, Thermo Fisher Scientific (Catalog No. 15140122); Cyclophosphamide (CTX): Sigma-Aldrich (Catalog No. C0768); Basic Fibroblast Growth Factor (BFGF): PeproTech (Catalog No. 100-18B); BFGF Inhibitor: R&D Systems (Catalog No. 7183-FB); CCK-8 Cell Viability Assay Kit: Dojindo Laboratories (Catalog No. CK04); Annexin V-FITC/PI Apoptosis Detection Kit: BD Biosciences (Catalog No. 556547); JC-1 Mitochondrial Membrane Potential Assay Kit: Abcam (Catalog No. ab113850); TUNEL Assay Kit: Roche (Catalog No. 11684795910); RIPA Lysis Buffer: Thermo Fisher Scientific (Catalog No. 89901); Protease and Phosphatase Inhibitor Cocktail: Sigma-Aldrich (Catalog No. P5726); PVDF Membranes: Millipore (Catalog No. IPVH00010); Enhanced Chemiluminescence (ECL) Detection Kit: Thermo Fisher Scientific (Catalog No. 32106); SERPINE1 Antibody: Abcam (Catalog No. ab125687); HIF-1α Antibody: Cell Signaling Technology (Catalog No. 3716); Nrf-2 Antibody: Abcam (Catalog No. ab137550); HO-1 Antibody: Cell Signaling Technology (Catalog No. 43966); Cyclin D1 Antibody: Cell Signaling Technology (Catalog No. 2978); CDK4 Antibody: Cell Signaling Technology (Catalog No. 12790); TGF-β Antibody: Abcam (Catalog No. ab92486); FSH Antibody: Abcam (Catalog No. ab151564); ER Antibody: Abcam (Catalog No. ab16660); β-actin Antibody: Cell Signaling Technology (Catalog No. 4970); HRP-Conjugated Secondary Antibodies: Cell Signaling Technology (Catalog No. 7074 for anti-rabbit, 7076 for anti-mouse); TRIzol Reagent: Thermo Fisher Scientific (Catalog No. 15596026); cDNA Synthesis Kit: Thermo Fisher Scientific (Catalog No. 4368814); SYBR Green PCR Master Mix: Thermo Fisher Scientific (Catalog No. A25742); Triton X-100: Sigma-Aldrich (Catalog No. T8787); Bovine Serum Albumin (BSA): Sigma-Aldrich (Catalog No. A9418); DAPI Stain: Thermo Fisher Scientific (Catalog No. D1306); Fluorescent Secondary Antibodies: Thermo Fisher Scientific (Catalog Nos. .. A-11001 and A-11008); Illumina TruSeq RNA Sample Preparation Kit: Illumina (Catalog No. RS-122-2001); Agilent RNA 6000 Nano Kit: Agilent Technologies (Catalog No. 5067 − 1511); Microplate reader (Super Max3100); Fluorescence microscope (Axio Vert A1);Flow cytometer (FASVERSE).

    SYBR Green Assay:

    Article Title: BFGF mitigates CTX-induced ovarian cytotoxicity via SERPINE1/HIF-1 and Nrf-2/HO-1 signaling pathways
    Article Snippet: .. DMEM/F12 Medium: Gibco, Thermo Fisher Scientific (Catalog No. 11330032); Fetal Bovine Serum (FBS): Gibco, Thermo Fisher Scientific (Catalog No. 10099141); Penicillin-Streptomycin: Gibco, Thermo Fisher Scientific (Catalog No. 15140122); Cyclophosphamide (CTX): Sigma-Aldrich (Catalog No. C0768); Basic Fibroblast Growth Factor (BFGF): PeproTech (Catalog No. 100-18B); BFGF Inhibitor: R&D Systems (Catalog No. 7183-FB); CCK-8 Cell Viability Assay Kit: Dojindo Laboratories (Catalog No. CK04); Annexin V-FITC/PI Apoptosis Detection Kit: BD Biosciences (Catalog No. 556547); JC-1 Mitochondrial Membrane Potential Assay Kit: Abcam (Catalog No. ab113850); TUNEL Assay Kit: Roche (Catalog No. 11684795910); RIPA Lysis Buffer: Thermo Fisher Scientific (Catalog No. 89901); Protease and Phosphatase Inhibitor Cocktail: Sigma-Aldrich (Catalog No. P5726); PVDF Membranes: Millipore (Catalog No. IPVH00010); Enhanced Chemiluminescence (ECL) Detection Kit: Thermo Fisher Scientific (Catalog No. 32106); SERPINE1 Antibody: Abcam (Catalog No. ab125687); HIF-1α Antibody: Cell Signaling Technology (Catalog No. 3716); Nrf-2 Antibody: Abcam (Catalog No. ab137550); HO-1 Antibody: Cell Signaling Technology (Catalog No. 43966); Cyclin D1 Antibody: Cell Signaling Technology (Catalog No. 2978); CDK4 Antibody: Cell Signaling Technology (Catalog No. 12790); TGF-β Antibody: Abcam (Catalog No. ab92486); FSH Antibody: Abcam (Catalog No. ab151564); ER Antibody: Abcam (Catalog No. ab16660); β-actin Antibody: Cell Signaling Technology (Catalog No. 4970); HRP-Conjugated Secondary Antibodies: Cell Signaling Technology (Catalog No. 7074 for anti-rabbit, 7076 for anti-mouse); TRIzol Reagent: Thermo Fisher Scientific (Catalog No. 15596026); cDNA Synthesis Kit: Thermo Fisher Scientific (Catalog No. 4368814); SYBR Green PCR Master Mix: Thermo Fisher Scientific (Catalog No. A25742); Triton X-100: Sigma-Aldrich (Catalog No. T8787); Bovine Serum Albumin (BSA): Sigma-Aldrich (Catalog No. A9418); DAPI Stain: Thermo Fisher Scientific (Catalog No. D1306); Fluorescent Secondary Antibodies: Thermo Fisher Scientific (Catalog Nos. .. A-11001 and A-11008); Illumina TruSeq RNA Sample Preparation Kit: Illumina (Catalog No. RS-122-2001); Agilent RNA 6000 Nano Kit: Agilent Technologies (Catalog No. 5067 − 1511); Microplate reader (Super Max3100); Fluorescence microscope (Axio Vert A1);Flow cytometer (FASVERSE).

    Polymerase Chain Reaction:

    Article Title: BFGF mitigates CTX-induced ovarian cytotoxicity via SERPINE1/HIF-1 and Nrf-2/HO-1 signaling pathways
    Article Snippet: .. DMEM/F12 Medium: Gibco, Thermo Fisher Scientific (Catalog No. 11330032); Fetal Bovine Serum (FBS): Gibco, Thermo Fisher Scientific (Catalog No. 10099141); Penicillin-Streptomycin: Gibco, Thermo Fisher Scientific (Catalog No. 15140122); Cyclophosphamide (CTX): Sigma-Aldrich (Catalog No. C0768); Basic Fibroblast Growth Factor (BFGF): PeproTech (Catalog No. 100-18B); BFGF Inhibitor: R&D Systems (Catalog No. 7183-FB); CCK-8 Cell Viability Assay Kit: Dojindo Laboratories (Catalog No. CK04); Annexin V-FITC/PI Apoptosis Detection Kit: BD Biosciences (Catalog No. 556547); JC-1 Mitochondrial Membrane Potential Assay Kit: Abcam (Catalog No. ab113850); TUNEL Assay Kit: Roche (Catalog No. 11684795910); RIPA Lysis Buffer: Thermo Fisher Scientific (Catalog No. 89901); Protease and Phosphatase Inhibitor Cocktail: Sigma-Aldrich (Catalog No. P5726); PVDF Membranes: Millipore (Catalog No. IPVH00010); Enhanced Chemiluminescence (ECL) Detection Kit: Thermo Fisher Scientific (Catalog No. 32106); SERPINE1 Antibody: Abcam (Catalog No. ab125687); HIF-1α Antibody: Cell Signaling Technology (Catalog No. 3716); Nrf-2 Antibody: Abcam (Catalog No. ab137550); HO-1 Antibody: Cell Signaling Technology (Catalog No. 43966); Cyclin D1 Antibody: Cell Signaling Technology (Catalog No. 2978); CDK4 Antibody: Cell Signaling Technology (Catalog No. 12790); TGF-β Antibody: Abcam (Catalog No. ab92486); FSH Antibody: Abcam (Catalog No. ab151564); ER Antibody: Abcam (Catalog No. ab16660); β-actin Antibody: Cell Signaling Technology (Catalog No. 4970); HRP-Conjugated Secondary Antibodies: Cell Signaling Technology (Catalog No. 7074 for anti-rabbit, 7076 for anti-mouse); TRIzol Reagent: Thermo Fisher Scientific (Catalog No. 15596026); cDNA Synthesis Kit: Thermo Fisher Scientific (Catalog No. 4368814); SYBR Green PCR Master Mix: Thermo Fisher Scientific (Catalog No. A25742); Triton X-100: Sigma-Aldrich (Catalog No. T8787); Bovine Serum Albumin (BSA): Sigma-Aldrich (Catalog No. A9418); DAPI Stain: Thermo Fisher Scientific (Catalog No. D1306); Fluorescent Secondary Antibodies: Thermo Fisher Scientific (Catalog Nos. .. A-11001 and A-11008); Illumina TruSeq RNA Sample Preparation Kit: Illumina (Catalog No. RS-122-2001); Agilent RNA 6000 Nano Kit: Agilent Technologies (Catalog No. 5067 − 1511); Microplate reader (Super Max3100); Fluorescence microscope (Axio Vert A1);Flow cytometer (FASVERSE).

    Staining:

    Article Title: BFGF mitigates CTX-induced ovarian cytotoxicity via SERPINE1/HIF-1 and Nrf-2/HO-1 signaling pathways
    Article Snippet: .. DMEM/F12 Medium: Gibco, Thermo Fisher Scientific (Catalog No. 11330032); Fetal Bovine Serum (FBS): Gibco, Thermo Fisher Scientific (Catalog No. 10099141); Penicillin-Streptomycin: Gibco, Thermo Fisher Scientific (Catalog No. 15140122); Cyclophosphamide (CTX): Sigma-Aldrich (Catalog No. C0768); Basic Fibroblast Growth Factor (BFGF): PeproTech (Catalog No. 100-18B); BFGF Inhibitor: R&D Systems (Catalog No. 7183-FB); CCK-8 Cell Viability Assay Kit: Dojindo Laboratories (Catalog No. CK04); Annexin V-FITC/PI Apoptosis Detection Kit: BD Biosciences (Catalog No. 556547); JC-1 Mitochondrial Membrane Potential Assay Kit: Abcam (Catalog No. ab113850); TUNEL Assay Kit: Roche (Catalog No. 11684795910); RIPA Lysis Buffer: Thermo Fisher Scientific (Catalog No. 89901); Protease and Phosphatase Inhibitor Cocktail: Sigma-Aldrich (Catalog No. P5726); PVDF Membranes: Millipore (Catalog No. IPVH00010); Enhanced Chemiluminescence (ECL) Detection Kit: Thermo Fisher Scientific (Catalog No. 32106); SERPINE1 Antibody: Abcam (Catalog No. ab125687); HIF-1α Antibody: Cell Signaling Technology (Catalog No. 3716); Nrf-2 Antibody: Abcam (Catalog No. ab137550); HO-1 Antibody: Cell Signaling Technology (Catalog No. 43966); Cyclin D1 Antibody: Cell Signaling Technology (Catalog No. 2978); CDK4 Antibody: Cell Signaling Technology (Catalog No. 12790); TGF-β Antibody: Abcam (Catalog No. ab92486); FSH Antibody: Abcam (Catalog No. ab151564); ER Antibody: Abcam (Catalog No. ab16660); β-actin Antibody: Cell Signaling Technology (Catalog No. 4970); HRP-Conjugated Secondary Antibodies: Cell Signaling Technology (Catalog No. 7074 for anti-rabbit, 7076 for anti-mouse); TRIzol Reagent: Thermo Fisher Scientific (Catalog No. 15596026); cDNA Synthesis Kit: Thermo Fisher Scientific (Catalog No. 4368814); SYBR Green PCR Master Mix: Thermo Fisher Scientific (Catalog No. A25742); Triton X-100: Sigma-Aldrich (Catalog No. T8787); Bovine Serum Albumin (BSA): Sigma-Aldrich (Catalog No. A9418); DAPI Stain: Thermo Fisher Scientific (Catalog No. D1306); Fluorescent Secondary Antibodies: Thermo Fisher Scientific (Catalog Nos. .. A-11001 and A-11008); Illumina TruSeq RNA Sample Preparation Kit: Illumina (Catalog No. RS-122-2001); Agilent RNA 6000 Nano Kit: Agilent Technologies (Catalog No. 5067 − 1511); Microplate reader (Super Max3100); Fluorescence microscope (Axio Vert A1);Flow cytometer (FASVERSE).

    Article Title: Composition analysis of extracellular vesicle-like particles derived from Hedyotis diffusa Willd and their effects on apoptosis in hepatocellular carcinoma Huh-7 cells.
    Article Snippet: As significant facilitators of intercellular communication, extracellular vesicles transport various bioactive molecules that contribute to the modulation of the tumor microenvironment.. CHM-EVLPs can markedly influence the biological behavior of tumor cells, exhibiting antitumor effects.. In this study, EV-like particles derived from Hedyotis diffusa Willd (HDW-EVLPs) were successfully isolated using the 3D FloTrix® vivaEXO exosome harvesting system.

    Transfection:

    Article Title: Hyperglycemia drives lmmune evasion in colorectal cancer through VISTA stabilization
    Article Snippet: GST-ERK2 (ab155812), ATP-γ-S, and p-Nitrobenzylmesylate (PNBM) were purchased from Abcam. .. The Lipo8000 transfection reagent was purchased from Beyotime, while the CCK-8 cell viability assay kit was acquired from DOJINDO. .. Anti-FLAG ® M2 Magnetic Beads, S02 and streptozotocin (STZ) were supplied by Sigma.

    Article Title: Hyperglycemia drives lmmune evasion in colorectal cancer through VISTA stabilization.
    Article Snippet: GST‐ERK2 129 (ab155812), ATP-γ-S, and p-Nitrobenzylmesylate (PNBM) were purchased 130 from Abcam. .. The Lipo8000 transfection reagent was purchased from 131 Beyotime, while the CCK-8 cell viability assay kit was acquired from 132 DOJINDO. .. Anti-FLAG® M2 Magnetic Beads, S02 and streptozotocin (STZ) 133 were supplied by Sigma.

    Isolation:

    Article Title: Composition analysis of extracellular vesicle-like particles derived from Hedyotis diffusa Willd and their effects on apoptosis in hepatocellular carcinoma Huh-7 cells.
    Article Snippet: As significant facilitators of intercellular communication, extracellular vesicles transport various bioactive molecules that contribute to the modulation of the tumor microenvironment.. CHM-EVLPs can markedly influence the biological behavior of tumor cells, exhibiting antitumor effects.. In this study, EV-like particles derived from Hedyotis diffusa Willd (HDW-EVLPs) were successfully isolated using the 3D FloTrix® vivaEXO exosome harvesting system.

    Transmission Assay:

    Article Title: Composition analysis of extracellular vesicle-like particles derived from Hedyotis diffusa Willd and their effects on apoptosis in hepatocellular carcinoma Huh-7 cells.
    Article Snippet: As significant facilitators of intercellular communication, extracellular vesicles transport various bioactive molecules that contribute to the modulation of the tumor microenvironment.. CHM-EVLPs can markedly influence the biological behavior of tumor cells, exhibiting antitumor effects.. In this study, EV-like particles derived from Hedyotis diffusa Willd (HDW-EVLPs) were successfully isolated using the 3D FloTrix® vivaEXO exosome harvesting system.

    Microscopy:

    Article Title: Composition analysis of extracellular vesicle-like particles derived from Hedyotis diffusa Willd and their effects on apoptosis in hepatocellular carcinoma Huh-7 cells.
    Article Snippet: As significant facilitators of intercellular communication, extracellular vesicles transport various bioactive molecules that contribute to the modulation of the tumor microenvironment.. CHM-EVLPs can markedly influence the biological behavior of tumor cells, exhibiting antitumor effects.. In this study, EV-like particles derived from Hedyotis diffusa Willd (HDW-EVLPs) were successfully isolated using the 3D FloTrix® vivaEXO exosome harvesting system.

    Fluorescence:

    Article Title: Composition analysis of extracellular vesicle-like particles derived from Hedyotis diffusa Willd and their effects on apoptosis in hepatocellular carcinoma Huh-7 cells.
    Article Snippet: As significant facilitators of intercellular communication, extracellular vesicles transport various bioactive molecules that contribute to the modulation of the tumor microenvironment.. CHM-EVLPs can markedly influence the biological behavior of tumor cells, exhibiting antitumor effects.. In this study, EV-like particles derived from Hedyotis diffusa Willd (HDW-EVLPs) were successfully isolated using the 3D FloTrix® vivaEXO exosome harvesting system.

    Imaging:

    Article Title: Composition analysis of extracellular vesicle-like particles derived from Hedyotis diffusa Willd and their effects on apoptosis in hepatocellular carcinoma Huh-7 cells.
    Article Snippet: As significant facilitators of intercellular communication, extracellular vesicles transport various bioactive molecules that contribute to the modulation of the tumor microenvironment.. CHM-EVLPs can markedly influence the biological behavior of tumor cells, exhibiting antitumor effects.. In this study, EV-like particles derived from Hedyotis diffusa Willd (HDW-EVLPs) were successfully isolated using the 3D FloTrix® vivaEXO exosome harvesting system.

    Flow Cytometry:

    Article Title: Composition analysis of extracellular vesicle-like particles derived from Hedyotis diffusa Willd and their effects on apoptosis in hepatocellular carcinoma Huh-7 cells.
    Article Snippet: As significant facilitators of intercellular communication, extracellular vesicles transport various bioactive molecules that contribute to the modulation of the tumor microenvironment.. CHM-EVLPs can markedly influence the biological behavior of tumor cells, exhibiting antitumor effects.. In this study, EV-like particles derived from Hedyotis diffusa Willd (HDW-EVLPs) were successfully isolated using the 3D FloTrix® vivaEXO exosome harvesting system.

    Real-time Polymerase Chain Reaction:

    Article Title: Composition analysis of extracellular vesicle-like particles derived from Hedyotis diffusa Willd and their effects on apoptosis in hepatocellular carcinoma Huh-7 cells.
    Article Snippet: As significant facilitators of intercellular communication, extracellular vesicles transport various bioactive molecules that contribute to the modulation of the tumor microenvironment.. CHM-EVLPs can markedly influence the biological behavior of tumor cells, exhibiting antitumor effects.. In this study, EV-like particles derived from Hedyotis diffusa Willd (HDW-EVLPs) were successfully isolated using the 3D FloTrix® vivaEXO exosome harvesting system.



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